There are many strains of zebrafish often used in the lab, many of them are used as wild type. Van den Bos and his colleagues (PLoS ONE, 2017) tested differences in activity and habituation of two often used “wild type” strains, the AB strain and the Tupfel long-fin.
They found that strain differences are not just morphological, emphasizing the need to be careful when comparing physiological and behavioral results between studies.
Functional implications of strain differences were tested with a light-dark test in DanioVision. After 20 minutes of acclimation, zebrafish underwent a 10 minutes dark – 10 minutes bright light (3000 lux) – 10 minutes dark regime. With EthoVision XT distance moved, maximum velocity during dark to light transitions, and changes in distance moved during light to dark transitions were measured.
DanioVision was also used to measure startle response. For this, the Observation Chamber was fitted with the Tapping Device for an acoustic/vibrational stimulus to elicit the startle response. With the intensity setting at 6, different stimuli intervals were used. The startle response is shown as a short burst of activity, so researchers used maximum velocity as parameter to measure it.
Bos, R. van den; Mes, W.; Galligani, P.; Heil, A.; Zethof, J.; Flik, G.; Gorissen, M. (2017). Further characterisation of differences between TL and AB zebrafish (Danio rerio): Gene expression, physiology and behaviour at day 5 of the larval stage. PLoS ONE, 12 (4), e0175420.