• Toxicity of quantum dots

To better test the effects on locomotor activity in response to certain compounds, zebrafish larvae can be loaded into multi-well plates. For example, Duan et al. (2013) investigated the developmental toxicity of quantum dots in larvae using 24-well plates in a DanioVision system. 

Quantum dots

Quantum dots are nanoparticles made of a semiconductor material, that have unique optical properties: the wavelength (and thus color) of their light emission depends on their size rather than the material they are made of. As a result, they are of great interest for fields such as biological imaging, medical diagnostics, drug delivery, and gene therapy.

Toxicity testing

Certain quantum dots may be toxic, limiting their usefulness. Duan investigated a specific type of quantum dots called Cadmium Telluride (CdTe) in a zebrafish study.

The researchers looked at activity levels of 6 dpf larvae swimming in visible light in a DanioVision Observation Chamber. Using EthoVision XT, they found that the total swimming distance was suppressed in treated larvae in a dose-dependent manner.

In a light-dark experiment (10-minute bouts of alternating light conditions), the control group showed the typical increased activity levels during dark phases. In comparison, the treated larvae showed significant hypo-activity.

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Read more about this study on our blog

Zebrafish research with DanioVision

Larvae activity and movement patterns are basic measurements used in many studies. They can reveal information on stereotypic and epileptic behaviors, circadian rhythmicity, motor control, movement disorders, neural development, and more.

DanioVision is a complete system designed for exactly these types of experiments with zebrafish larvae, and is often used in studies related to drug development, safety pharmacology, behavioral genetics, and circadian rhythmicity.


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Reference

Duan, J.; Yu, Y.; Li, Y.; Yu, Y.; Li, Y.; Huang, P.; Zhou, X.; Peng, S.; Sun, Z. (2013). Developmental toxicity of CdTe QDs in zebrafish embryos and larvae. Journal of Nanoparticle Research15, 1700.