• Strain differences

There are many strains of zebrafish often used in the lab, many of them are used as wild type. Van den Bos and his colleagues (PLoS ONE, 2017) tested differences in activity and habituation of two often used “wild type” strains, the AB strain and the Tupfel long-fin.

They found that strain differences are not just morphological, emphasizing the need to be careful when comparing physiological and behavioral results between studies.

Light-dark test

Functional implications of strain differences were tested with a light-dark test in DanioVision. After 20 minutes of acclimation, zebrafish underwent a 10 minutes dark – 10 minutes bright light (3000 lux) – 10 minutes dark regime. With EthoVision XT distance moved, maximum velocity during dark to light transitions, and changes in distance moved during light to dark transitions were measured.

Startle response

DanioVision was also used to measure startle response. For this, the Observation Chamber was fitted with the Tapping Device for an acoustic/vibrational stimulus to elicit the startle response. With the intensity setting at 6, different stimuli intervals were used. The startle response is shown as a short burst of activity, so researchers used maximum velocity as parameter to measure it.

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Zebrafish research with DanioVision

Larvae activity and movement patterns are basic measurements used in many studies. They can reveal information on stereotypic and epileptic behaviors, circadian rhythmicity, motor control, movement disorders, neural development, and more.

DanioVision is a complete system designed for exactly these types of experiments with zebrafish larvae, and is often used in studies related to drug development, safety pharmacology, behavioral genetics, and circadian rhythmicity.


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Reference

Bos, R. van den; Mes, W.; Galligani, P.; Heil, A.; Zethof, J.; Flik, G.; Gorissen, M. (2017). Further characterisation of differences between TL and AB zebrafish (Danio rerio): Gene expression, physiology and behaviour at day 5 of the larval stage. PLoS ONE12 (4), e0175420.